Páez X, Hernández L
Laboratory of Behavioral Physiology, School of Medicine, Universidad de los Andes, Mérida, Venezuela.
J Chromatogr B Biomed Sci Appl. 1998 Dec 11;720(1-2):33-8. doi: 10.1016/s0378-4347(98)00417-4.
Plasma serotonin (5-HT) active pool was monitored in male volunteers by intravenous microdialysis coupled to HPLC-EC with 98.6% efficient probes. 5-HT was monitored from 60 min before to 360 min after an oral dose of fluoxetine, a 5-HT uptake inhibitor, or vehicle. The basal values were within nanomolar range (0.55 to 4.6 ng/ml). After administration of fluoxetine, there was a significant increment of 5-HT with respect to controls. These results showed that intravenous microdialysis is an alternative efficient technique to monitor endogenous unbound 5-HT changes in plasma without extracting blood or sample pretreatment procedures before the chemical analysis.
通过高效能为98.6%的探针，利用静脉微透析结合高效液相色谱-电化学检测法，对男性志愿者血浆血清素（5-羟色胺，5-HT）的活性池进行监测。在口服5-羟色胺摄取抑制剂氟西汀或赋形剂前60分钟至给药后360分钟对5-羟色胺进行监测。基础值在纳摩尔范围内（0.55至4.6纳克/毫升）。给予氟西汀后，与对照组相比，5-羟色胺显著增加。这些结果表明，静脉微透析是一种替代的有效技术，可在不进行血液提取或化学分析前样品预处理程序的情况下，监测血浆中内源性游离5-羟色胺的变化。
