Posteraro B, Sanguinetti M, Garcovich A, Ardito F, Zampetti A, Masucci L, Sbordoni G, Cerimele D, Fadda G
Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Rome,Italy.
Br J Dermatol. 1998 Nov;139(5):872-6. doi: 10.1046/j.1365-2133.1998.02516.x.
In this paper, we report a patient in whom Mycobacterium marinum sporotrichoid infection was diagnosed using polymerase chain reaction (PCR) amplification of the 16S rRNA gene and subsequent analysis of the amplified product in a reverse cross-blot hybridization assay with mycobacterial species-specific probes. This molecular method allowed us rapidly to detect and identify this organism directly in the patient's lesional skin biopsy rather than in cultures in conventional media. The identification provided by PCR-reverse cross-blot hybridization assay was confirmed by examination of the morphological and biochemical features and by high-performance liquid chromatography analysis of mycolic acid from the clinical isolate, suggesting the validity of our molecular approach.
在本文中，我们报告了一名患者，通过对16S rRNA基因进行聚合酶链反应（PCR）扩增，并随后使用分枝杆菌属特异性探针进行反向杂交印迹分析来检测扩增产物，从而诊断出该患者患有海分枝杆菌孢子丝菌样感染。这种分子方法使我们能够直接在患者的病变皮肤活检组织中快速检测和鉴定该病原体，而无需在传统培养基中进行培养。通过对临床分离株的形态学和生化特征进行检查以及对分枝菌酸进行高效液相色谱分析，证实了PCR-反向杂交印迹分析所提供的鉴定结果，这表明我们的分子方法是有效的。
