基于细胞内荧光素荧光偏振测量的淋巴细胞活化制备人黑色素瘤诊断抗原
Preparation of a diagnostic antigen of human melanoma based on lymphocyte activation as measured by intracellular fluorescein fluorescence polarization.
作者信息
Avtalion N, Avtalion R, Tirosh R, Sheinberg A, Weinreb A, Avinoach I, Deutsch M
机构信息
The Jerome Schottenstein Cellscan Center for Early Detection of Cancer, Physics Department, Bar-Ilan University, Ramat-Gan, Israel.
出版信息
Cancer Detect Prev. 1999;23(1):64-71. doi: 10.1046/j.1525-1500.1999.09901.x.
The intracellular fluorescein fluorescence polarization (IFFP) test indicates that peripheral blood lymphocytes (PBL) of cancer patients display stimulatory sensitivity to a short incubation with specific tumor protein extracts. In this work, a human lymphocyte activation melanoma antigen (LAMA) was purified from supernatant of a human melanoma cell line (L1M1), which could specifically stimulate lymphocytes of melanoma patients. The results showed a significant stimulation of lymphocytes from healthy donors after incubation with phytohaemagglutinin (PHA), while no stimulation was observed after incubation with LAMA. On the other hand, lymphocytes from melanoma patients showed a significant stimulation with LAMA, while generally showing minor or no stimulation with PHA. Melanoma specificity of LAMA was demonstrated by no response in lymphocytes from patients of lung, colon, or breast cancer. The purified fraction is therefore considered to be a shared tissue-specific antigen which may be useful in immunodiagnosis and immunotherapy of melanoma.
细胞内荧光素荧光偏振（IFFP）试验表明，癌症患者的外周血淋巴细胞（PBL）对与特定肿瘤蛋白提取物短时间孵育表现出刺激敏感性。在这项研究中，从人黑色素瘤细胞系（L1M1）的上清液中纯化出一种人淋巴细胞激活黑色素瘤抗原（LAMA），它能特异性刺激黑色素瘤患者的淋巴细胞。结果显示，健康供体的淋巴细胞在与植物血凝素（PHA）孵育后受到显著刺激，而与LAMA孵育后未观察到刺激。另一方面，黑色素瘤患者的淋巴细胞在与LAMA孵育后受到显著刺激，而通常对PHA的刺激较小或无反应。肺癌、结肠癌或乳腺癌患者的淋巴细胞对LAMA无反应，证明了LAMA的黑色素瘤特异性。因此，纯化的组分被认为是一种共同的组织特异性抗原，可能在黑色素瘤的免疫诊断和免疫治疗中有用。
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