Merimsky O, Kaplan B, Deutsch M, Tirosh R, Weinreb A, Chaitchik S
Department of Oncology, Tel Aviv Sourasky Medical Center, Israel.
Cancer Detect Prev. 1997;21(2):167-77.
The effectiveness of detecting melanoma by measuring the intracellular fluorescein fluorescent polarization (IFFP) of patients' SCM (structuredness of the cytoplasmic matrix)-responding lymphocytes was examined. SCM-responding lymphocytes from 46 melanoma patients and 32 healthy volunteers were labeled with fluorescein diacetate and challenged with different stimuli, and the resulting polarization was determined. The polarizations (P) obtained upon stimulation with nothing (P-0), encephalitogenic factor (P-EF), phytohaemagglutinin (P-PHA), or melanoma antigen (P-MEL), and the ratios RR(ef) (P-EF/P-PHA) and RR(mel) (P-MEL/P-PHA) were lower for SCM-responding lymphocytes from the patients as a group than for those of the controls. The specificity and sensitivity of the IFFP tests (using cutoff values) to detect melanoma were 90.6 and 73.9%, respectively. The IFFP tests may facilitate the discrimination between melanoma patients and healthy subjects, and may be used in follow-up of patients with melanoma.
通过测量患者SCM（细胞质基质结构）反应性淋巴细胞的细胞内荧光素荧光偏振（IFFP）来检测黑色素瘤的有效性进行了研究。对46例黑色素瘤患者和32名健康志愿者的SCM反应性淋巴细胞用二醋酸荧光素进行标记，并用不同刺激物进行激发，然后测定产生的偏振情况。用无刺激物（P-0）、致脑炎因子（P-EF）、植物血凝素（P-PHA）或黑色素瘤抗原（P-MEL）刺激后得到的偏振值（P），以及RR(ef)（P-EF/P-PHA）和RR(mel)（P-MEL/P-PHA）比值，作为一个整体的患者组的SCM反应性淋巴细胞的值低于对照组。IFFP检测（使用临界值）检测黑色素瘤的特异性和敏感性分别为90.6%和73.9%。IFFP检测可能有助于区分黑色素瘤患者和健康受试者，并可用于黑色素瘤患者的随访。
