从外源整合体中克隆未知细胞侧翼DNA序列的策略。
Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants.
作者信息
Hui E K, Wang P C, Lo S J
机构信息
Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei, Taiwan.
出版信息
Cell Mol Life Sci. 1998 Dec;54(12):1403-11. doi: 10.1007/s000180050262.
Abstract
Many virus and transposon DNAs can integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.
摘要
许多病毒和转座子DNA能够整合到宿主基因组中。在本综述中，介绍了包括反向聚合酶链反应（IPCR）、新型Alu-PCR以及连接头或锚定PCR等技术，作为克隆整合子侧翼DNA区域的可能策略。还描述了靶向基因步移PCR、限制性位点PCR、捕获PCR、锅柄PCR和回飞镖DNA扩增。讨论了每种方法的原理、优点和局限性。
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