Hui E K, Wang P C, Lo S J
Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei, Taiwan.
Cell Mol Life Sci. 1998 Dec;54(12):1403-11. doi: 10.1007/s000180050262.
Many virus and transposon DNAs can integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.
许多病毒和转座子DNA能够整合到宿主基因组中。在本综述中,介绍了包括反向聚合酶链反应(IPCR)、新型Alu-PCR以及连接头或锚定PCR等技术,作为克隆整合子侧翼DNA区域的可能策略。还描述了靶向基因步移PCR、限制性位点PCR、捕获PCR、锅柄PCR和回飞镖DNA扩增。讨论了每种方法的原理、优点和局限性。