Słowińska-Klencka D, Klencki M, Sporny S, Lewiński A
Department of Thyroidology, Medical University of Lódź, Poland.
Anal Quant Cytol Histol. 1997 Dec;19(6):507-13.
To assess the possible role of karyometry in improving the diagnostic value of fine needle aspiration biopsy (FNAB) of the thyroid.
Thyrocyte nuclei were evaluated in routine slides obtained from 229 patients. The cytologic specimens were divided into seven groups according to the results of corresponding postoperative histopathologic examinations. Karyometric measurements comprised assessment of nuclear volume intersection area and perimeter as well as level of anisokaryosis, evaluated as standard deviations of those parameters in single slides. The karyometric evaluation was performed with a computerized image analysis system.
The size of thyrocyte nuclei increased from nontoxic nodular goiters through follicular adenomas, toxic nodular goiters treated with antithyroid drugs, lymphocytic thyroiditis, papillary and follicular carcinomas to anaplastic carcinomas. The observed differences between those groups were statistically significant, but there was marked overlapping. Our data indicate also that the age and sex of the patient influence the cytologic picture of nodular goiter. The evaluation of anisokaryosis increases the usefulness of karyometry in differentiating malignant neoplasms from benign lesions.
Karyometric analysis increases the probability of a correct diagnosis with FNAB examination.
评估细胞核测量术在提高甲状腺细针穿刺活检（FNAB）诊断价值方面的可能作用。
对从229例患者获取的常规载玻片上的甲状腺细胞核进行评估。根据相应术后组织病理学检查结果，将细胞学标本分为七组。细胞核测量包括评估核体积、交叉面积、周长以及核大小不等的程度，核大小不等程度通过单张载玻片上这些参数的标准差来评估。使用计算机图像分析系统进行细胞核测量评估。
甲状腺细胞核大小从非毒性结节性甲状腺肿、滤泡性腺瘤、用抗甲状腺药物治疗的毒性结节性甲状腺肿、淋巴细胞性甲状腺炎、乳头状癌和滤泡状癌到未分化癌逐渐增大。这些组之间观察到的差异具有统计学意义，但存在明显重叠。我们的数据还表明，患者的年龄和性别会影响结节性甲状腺肿的细胞学表现。核大小不等的评估增加了细胞核测量术在区分恶性肿瘤与良性病变方面的效用。
细胞核测量分析增加了FNAB检查正确诊断的概率。
