生物素偶联的多螯合剂
Biotin-conjugated polychelating agent.
作者信息
Torchilin V P
机构信息
Center for Imaging and Pharmaceutical Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129, USA.
出版信息
Bioconjug Chem. 1999 Jan-Feb;10(1):146-9. doi: 10.1021/bc9800567.
A procedure is described for preparing a polychelating agent-biotin conjugate through the interaction of biotin-maleimide with poly-L-lysine modified with multiple residues of diethylenetriaminepentaacetic acid and containing amino-terminal pyridylthio-propionate group. The conjugate can be easily loaded with multiple metal atoms, such as 111In, and preserves its ability to specifically interact with avidin (as avidin-agarose). Polychelate-biotin conjugate may be used for signal amplification in visualization and analytical protocols involving the application of the avidin-biotin system.
描述了一种通过生物素-马来酰亚胺与用二亚乙基三胺五乙酸的多个残基修饰并含有氨基末端吡啶硫基丙酸酯基团的聚-L-赖氨酸相互作用来制备多螯合剂-生物素缀合物的方法。该缀合物可以容易地负载多个金属原子，如铟-111，并保留其与抗生物素蛋白（如抗生物素蛋白-琼脂糖）特异性相互作用的能力。多螯合物-生物素缀合物可用于涉及抗生物素蛋白-生物素系统应用的可视化和分析方案中的信号放大。
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