一种用于评估恶性疟原虫感染的红细胞与人类合体滋养层结合能力的体外微量测定法。
An in vitro microassay to assess the ability of Plasmodium falciparum-infected erythrocytes to bind to the human syncytiotrophoblast.
作者信息
Maubert B, Riche D, Deloron P
机构信息
Institut National de la Santé et de la Recherche Médicale d'Epidémiologie U13/Institut de Médical et d'Epidémiologie Africaine, Hôpital Bichat-Claude Bernard, Paris, France.
出版信息
Am J Reprod Immunol. 1998 Dec;40(6):401-7. doi: 10.1111/j.1600-0897.1998.tb00425.x.
PROBLEM
Erythrocytes parasitized by matures stages of Plasmodium falciparum are frequently sequestered in human placenta. The consequences of this sequestration have been well described, but little is known about the mechanisms used by the parasite to concentrate in the placenta.
METHOD OF STUDY
We developed an in vitro assay to study their binding capacity to the human syncytiotrophoblast. Our cytoadherence test was scaled down, and each step of the assay was optimized to enhance the sensitivity of our model.
RESULTS
Cytoadherence assays between P. falciparum-infected erythrocytes and human trophoblasts are easily performed using low numbers of trophoblast cells. The process can also be used to carry out immunofluorescence and immunostaining techniques.
CONCLUSIONS
The test may be adapted to any kind of cell, is inexpensive, and allows the culture of virtually any kind of cell for several weeks.
问题
被恶性疟原虫成熟阶段寄生的红细胞经常滞留于人类胎盘。这种滞留的后果已有详尽描述，但对于寄生虫集中于胎盘所采用的机制却知之甚少。
研究方法
我们开发了一种体外试验来研究其与人合体滋养层细胞的结合能力。我们缩小了细胞黏附试验规模，并对试验的每个步骤进行了优化，以提高我们模型的敏感性。
结果
使用少量滋养层细胞即可轻松进行恶性疟原虫感染的红细胞与人滋养层细胞之间的细胞黏附试验。该方法还可用于进行免疫荧光和免疫染色技术。
结论
该试验可适用于任何类型的细胞，成本低廉，并且几乎能使任何类型的细胞培养数周。
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