Jiménez P, Cantón J, Concha A, Torres L M, Abril E, Real L M, García A, Garrido F, Ruiz-Cabello F
Dept. de Análisis Clínicos, Hospital Universitario Virgen de las Nieves, Universidad de Granada, Spain.
J Exp Clin Cancer Res. 1998 Sep;17(3):361-6.
We studied the incidence of microsatellite instability (MI) in lesions defined as cervical intraepithelial neoplasia (CIN). The human papillomavirus (HPV) status of the tissues was also determined. DNA from tissue samples and autologous lymphocytes were studied for five loci located within or adjacent to the DNA mismatch repair genes. Replicate errors were detected in 7 out of 47 (14.8%) samples of cervical tissue from 24 women. Our results indicate that the defect in DNA repair-associated genes does not appear to be necessary for the selection of clones which progress from dysplasia to carcinoma. Although HPV DNA of highly oncogenic types (16/18) was detected in most cervical lesions and may be an important factor for MI, we also detected MI in two loci in HPV-negative normal tissue, indicating that further events can also be involved in mismatch repair defects.
我们研究了被定义为宫颈上皮内瘤变（CIN）的病变中微卫星不稳定性（MI）的发生率。还确定了组织的人乳头瘤病毒（HPV）状态。对来自组织样本和自体淋巴细胞的DNA进行了位于DNA错配修复基因内部或附近的五个位点的研究。在24名女性的47份宫颈组织样本中的7份（14.8%）中检测到重复错误。我们的结果表明，DNA修复相关基因的缺陷对于从发育异常进展为癌的克隆选择似乎并非必要。虽然在大多数宫颈病变中检测到高致癌性类型（16/18）的HPV DNA，并且其可能是MI的一个重要因素，但我们在HPV阴性的正常组织中的两个位点也检测到了MI，这表明错配修复缺陷可能还涉及其他事件。
