Kim B, Shortridge R D, Seong C, Oh Y, Baek K, Yoon J
Institute of Genetic Engineering and Natural Sciences, Department of Genetic Engineering, Kyung Hee University, Yongin City, Korea.
Mol Cells. 1998 Dec 31;8(6):750-7.
We have isolated and characterized a novel Drosophila melanogaster gene (noe) that is specifically and abundantly expressed in the central nervous system (CNS). The gene, which maps to 74B on the left arm of third chromosome, encodes a protein of 74 amino acids with no significant similarity to known protein sequences. The deduced amino acid sequence of the gene product is rich in basic amino acids, especially the lysine, and contains five potential phosphorylation sites. The noe gene lacks introns and seems to produce two transcripts by alternative polyadenylations. The promoter region deduced from 5'-RACE analysis contains a sequence similar to the TATA-box consensus sequence. RNA blot analysis detected 1.0 kb noe transcripts that are expressed from the third-instar larval stage to the adult stage and which are predominantly found in the adult heads. In situ hybridizations to tissue sections showed that the gene is abundantly expressed in neuronal cell bodies as well as in the neurophiles of adult and larval CNS (brain, optic lobe, and thoracic ganglia of adults and larval brain).
我们已经分离并鉴定了一种新的果蝇基因（noe），该基因在中枢神经系统（CNS）中特异性且大量表达。该基因定位于第三条染色体左臂的74B位置，编码一种由74个氨基酸组成的蛋白质，与已知蛋白质序列无明显相似性。该基因产物的推导氨基酸序列富含碱性氨基酸，尤其是赖氨酸，并含有五个潜在的磷酸化位点。noe基因缺乏内含子，似乎通过可变聚腺苷酸化产生两种转录本。从5'-RACE分析推导的启动子区域包含一个与TATA框共有序列相似的序列。RNA印迹分析检测到1.0 kb的noe转录本，其从三龄幼虫阶段到成虫阶段均有表达，且主要存在于成虫头部。对组织切片的原位杂交显示，该基因在神经元细胞体以及成虫和幼虫中枢神经系统（成虫的脑、视叶和胸神经节以及幼虫脑）的神经纤维中大量表达。
