果蝇中编码蝶啶还原酶的基因的结构、染色体定位及表达

Structure, chromosomal localization, and expression of the Drosophila melanogaster gene encoding sepiapterin reductase.

作者信息

Seong C, Baek K, Yoon J

机构信息

Department and Institute of Genetic Engineering, Kyung Hee University, 449-701, Yongin City, South Korea.

出版信息

Gene. 2000 Sep 19;255(2):357-61. doi: 10.1016/s0378-1119(00)00314-0.

DOI:10.1016/s0378-1119(00)00314-0

PMID:11024296

Abstract

We have isolated and characterized a Drosophila melanogaster gene encoding the sepiapterin reductase (SR). The gene does not have introns. The 5'- and 3'-RACE analysis, which determined the transcription start point (tsp) and polyadenylation site, respectively, showed that the gene produces single mRNA species. The potential promoter region lacks distinct TATAAA or CCAAT box consensus sequences. RNA blot analysis revealed that the gene encodes a 1.4kb transcript that could be detected throughout development and in both heads and bodies of adults. The Drosophila SR gene maps to 15A on the X chromosome.

摘要

我们已经分离并鉴定了果蝇中一个编码蝶呤还原酶（SR）的基因。该基因没有内含子。分别确定转录起始点（tsp）和多聚腺苷酸化位点的5'-和3'-RACE分析表明，该基因产生单一的mRNA种类。潜在的启动子区域缺乏明显的TATAAA或CCAAT框共有序列。RNA印迹分析显示，该基因编码一个1.4kb的转录本，在整个发育过程以及成虫的头部和身体中都能检测到。果蝇SR基因定位于X染色体的15A位置。