Rubins J B, Paddock A H, Charboneau D, Berry A M, Paton J C, Janoff E N
Department of Medicine, Veterans Affairs Medical Center and University of Minnesota School of Medicine, Minneapolis, MN, 55417, USA.
Microb Pathog. 1998 Dec;25(6):337-42. doi: 10.1006/mpat.1998.0239.
The universal and highly conserved production of pneumolysin, the major pneumococcal cytolysin, among clinical isolates of Streptococcus pneumoniae and the previously reported association of pneumolysin production with increased pneumococcal adherence to respiratory epithelium in organ cultures suggest that this toxin might be important for nasopharyngeal colonization. We confirmed that pneumolysin-deficient mutant pneumococcal strains had decreased adherence to respiratory epithelial cells in vitro compared with their isogeneic wild-type strains. However, neither early nor sustained colonization by type 14 S. pneumoniae in an established murine model was dependent on bacterial production of pneumolysin. We conclude that pneumolysin production is not a major determinant of successful nasopharyngeal colonization by pneumococci.
在肺炎链球菌的临床分离株中，主要的肺炎球菌溶素——肺炎溶素的产生具有普遍性且高度保守，并且先前报道的肺炎溶素产生与器官培养中肺炎球菌对呼吸道上皮细胞黏附增加之间的关联表明，这种毒素可能对鼻咽部定植很重要。我们证实，与同基因野生型菌株相比，缺乏肺炎溶素的突变肺炎球菌菌株在体外对呼吸道上皮细胞的黏附减少。然而，在已建立的小鼠模型中，14型肺炎链球菌的早期或持续定植均不依赖于细菌产生肺炎溶素。我们得出结论，肺炎溶素的产生不是肺炎球菌成功进行鼻咽部定植的主要决定因素。
