Langlois M R, Delanghe J R, Steyaert S R, Everaert K C, De Buyzere M L
Department of Clinical Chemistry, University Hospital Gent, Belgium.
Clin Chem. 1999 Jan;45(1):118-22.
Recently, the Sysmex UF-100 flow cytometer was developed to automate urinalysis. We compared UF-100 test results with those of an automated dipstick reader. A cross-check of UF-100, dipstick, and microscopic sediment data was performed in 1001 urine samples. Good agreements (P <0.001) were obtained between UF-100 and dipstick data for erythrocytes (r = 0.636) and leukocytes (r = 0.785). Even in urine with low conductivity, the UF-100 could detect lysed erythrocytes. The UF-100 bacterial count was higher among nitrite-positive urine samples (P <0.0001) and was positively correlated with the UF-100 leukocyte count (r = 0.745; P <0.001). In stored urine (24 h), bacterial counts increased, whereas the forward light scatter of leukocytes decreased (P <0.01). Casts and yeast cells reported by the UF-100 should be confirmed by microscopic review because false positives occurred. We suggest that a computer-assisted cross-check of UF-100 and dipstick data allows a clinically acceptable sieving system to reduce the workload of microscopic sediment urinalysis.
最近，Sysmex UF - 100流式细胞仪被开发用于尿液分析自动化。我们将UF - 100的检测结果与自动试纸读数器的结果进行了比较。对1001份尿液样本进行了UF - 100、试纸和显微镜下沉淀物数据的交叉核对。UF - 100与试纸检测红细胞（r = 0.636）和白细胞（r = 0.785）的数据之间具有良好的一致性（P <0.001）。即使在低电导率的尿液中，UF - 100也能检测到溶解的红细胞。在亚硝酸盐阳性的尿液样本中，UF - 100的细菌计数更高（P <0.0001），并且与UF - 100白细胞计数呈正相关（r = 0.745；P <0.001）。在储存的尿液（24小时）中，细菌计数增加，而白细胞的前向光散射减少（P <0.01）。UF - 100报告的管型和酵母细胞应通过显微镜复查来确认，因为会出现假阳性。我们建议对UF - 100和试纸数据进行计算机辅助交叉核对，以建立一个临床可接受的筛选系统，从而减少显微镜下沉淀物尿液分析的工作量。
