Del Principe D, Mancuso G, Menichelli A, Maretto G, Sabetta G
Thromb Haemost. 1976 Jun 30;35(3):712-6.
The authors compared the oxygen consumption in platelets from the umbilical cord blood of 36 healthy newborn infants with that of 27 adult subjects, before and after thrombin addition (1.67 U/ml). Oxygen consumption at rest was 6 mumol/10(9)/min in adult control platelets and 5.26 in newborn infants. The burst in oxygen consumption after thrombin addition was 26.30 mumol/10(9)/min in adults and 24.90 in infants. Dinitrophenol did not inhibit the burst of O2 consumption in platelets in 8 out of 10 newborn infants, while the same concentration caused a decrease in 9 out of 10 adult subjects. Deoxyglucose inhibited the burst in O2 consumption in newborn infant and adult platelets by about 50%. KCN at the concentration of 10(-4) M completely inhibited basal oxygen consumption but did not completely inhibit the burst after thrombin. At the concentration of 10(-3) M, it inhibited both basal O2 consumption and the burst in infants and adult subjects.
作者比较了36名健康新生儿脐带血中血小板的氧消耗量与27名成年受试者的血小板在添加凝血酶（1.67 U/ml）前后的氧消耗量。成年对照血小板静息时的氧消耗量为6 μmol/10⁹/分钟，新生儿为5.26 μmol/10⁹/分钟。添加凝血酶后氧消耗量的激增在成年人中为26.30 μmol/10⁹/分钟，在婴儿中为24.90 μmol/10⁹/分钟。二硝基苯酚在10名新生儿中有8名未抑制血小板中氧消耗的激增，而相同浓度在10名成年受试者中有9名导致氧消耗减少。脱氧葡萄糖使新生儿和成年血小板中氧消耗的激增受到约50%的抑制。浓度为10⁻⁴ M的KCN完全抑制了基础氧消耗，但未完全抑制凝血酶后的激增。在10⁻³ M的浓度下，它抑制了婴儿和成年受试者的基础氧消耗和激增。
