Poobalasingam N
Br J Anaesth. 1976 Oct;48(10):953-6. doi: 10.1093/bja/48.10.953.
A gas chromatographic method for the analysis of chloroform in blood has been developed in which the anaesthetic agent is extracted into carbon disulphide and trichlorethylene is used as an internal standard. Injection of the extract into a chromatograph resulted in a chloroform peak eluted after 165 s and a trichloroethylene peak at 310 s, both of which are much later than the solvent peak which is completely eluted in 100 s. The mean percentage recovery of chloroform from blood in the range of 1.4--38.12 mg/100 ml was 98.8+/-1.32. This is an improvement on all previous methods described for the estimation of chloroform.
已开发出一种用于分析血液中氯仿的气相色谱法，该方法是将麻醉剂萃取到二硫化碳中，并使用三氯乙烯作为内标。将萃取液注入色谱仪后，氯仿峰在165秒时洗脱，三氯乙烯峰在310秒时洗脱，这两个峰均比在100秒时完全洗脱的溶剂峰晚得多。氯仿在血液中的浓度范围为1.4--38.12毫克/100毫升时，其平均回收率为98.8±1.32%。这比以往描述的所有氯仿估算方法都有所改进。
