Lane F, Goff P, McGuffin R, Eichner E R, Hillman R S
Br J Haematol. 1976 Nov;34(3):489-500. doi: 10.1111/j.1365-2141.1976.tb03595.x.
Folate metabolism was studied in normal, folate-deficient and alcoholic man by tracer measurements of plasma clearance, urinary excretion, tissue storage and release of folate using both [3H]pteroylglutamic acid (3H-PteGlu) and 14C-methyl-H4PteGlu. Alcohol ingestion did not adversely affect tissue uptake of folates. Whether in normal or folate deficient subjects, the relative clearance rates of 3H-PteGlu and 14C-methyl-H4PteGlu were maintained in the face of alcohol ingestion and there was no evidence of increased urinary loss of intact vitamin or labelled breakdown products. As measured by the flushing technique, the rate of storage or tissue binding of 3H-PteGlu was not influenced by folate deficiency, folate store depletion or alcohol ingestion. However, alcohol may retard the release of methyl-H4PteGlu from tissue stores to plasma. A significantly greater recovery of 14C-methyl-H4PteGly with flush was observed in those normal subjects who ingested alcohol for 6 d. A partial block in the rate of release of tissue folate stores would be a possible mechanism behind the rapid depression in serum methyl-H4PteGlu levels and early induction of megaloblastic erythropoiesis which has been observed following acute alcohol ingestion.
通过使用[3H]蝶酰谷氨酸（3H-PteGlu）和14C-甲基-H4PteGlu对血浆清除率、尿排泄、组织储存和叶酸释放进行示踪测量，研究了正常、叶酸缺乏和酗酒男性的叶酸代谢。饮酒对组织摄取叶酸没有不利影响。无论是正常受试者还是叶酸缺乏受试者，面对饮酒时，3H-PteGlu和14C-甲基-H4PteGlu的相对清除率均保持不变，且没有证据表明完整维生素或标记分解产物的尿流失增加。通过冲洗技术测量，3H-PteGlu的储存或组织结合率不受叶酸缺乏、叶酸储存耗竭或饮酒的影响。然而，酒精可能会延缓甲基-H4PteGlu从组织储存释放到血浆中的过程。在连续6天饮酒的正常受试者中，观察到冲洗后14C-甲基-H4PteGly的回收率显著更高。组织叶酸储存释放速率的部分阻滞可能是急性饮酒后血清甲基-H4PteGlu水平迅速下降和巨幼细胞性红细胞生成早期诱导背后的一种可能机制。
