Glinoer D, Robbins J
Ann Endocrinol (Paris). 1978;39(2):141-2.
Serum thyroxine-binding globulin (TBG), the major plasma transport protein for thyroid hormones in man, was shown to be altered under the influence of estrogen and in hypothyroidism. In order to study these alterations, we used an animal model. Synthesis of TBG was demonstrated in hepatocytes isolated from adult Rhesus monkeys, and in a monkey hepatocarcinoma continuous cell culture line (NCLP-6-E). When the hepatocytes were obtained from monkeys pretreated with beta-estradiol (E2), a specific 2.5-2.9 fold increase of TBG synthesis and secretion was shown; similar data were obtained with the tumor line. Furthermore, an increased TBG production was shown with these cells when T4 (from 10(-14) to 10(-11) M) was added to the culture medium. The in vitro results were correlated with in vivo data obtained by investigating the metabolism of TBG after iv injection of tracer doses of purified, radiolabelled TBG to normal, E2-treated and thyroidectomized monkeys. The main effect of estrogen administration was a marked increase of the production rate. During hypothyroidism, the catabolism and the production rate of TBG were decreased. In both conditions, there were significant changes in the distribution volume of TBG, the physiologic relevance of which remains to be investigated.
血清甲状腺素结合球蛋白（TBG）是人体内甲状腺激素的主要血浆转运蛋白，已证实在雌激素影响下及甲状腺功能减退时会发生改变。为研究这些改变，我们使用了一种动物模型。在从成年恒河猴分离的肝细胞以及一种猴肝癌连续细胞系（NCLP - 6 - E）中证实了TBG的合成。当肝细胞取自用β - 雌二醇（E2）预处理过的猴子时，TBG的合成与分泌显示出特异性地增加了2.5至2.9倍；肿瘤细胞系也得到了类似的数据。此外，当向培养基中添加T4（浓度从10^(-14)至10^(-11) M）时，这些细胞的TBG产生量增加。体外实验结果与体内实验数据相关，体内实验数据是通过向正常、E2处理过的和甲状腺切除的猴子静脉注射示踪剂量的纯化放射性标记TBG后研究TBG的代谢获得的。给予雌激素的主要作用是显著提高产生率。在甲状腺功能减退时，TBG的分解代谢和产生率降低。在这两种情况下，TBG的分布容积都有显著变化，其生理相关性仍有待研究。
