Makarova R A, Chaĭkovskaia S M, Semenov S M, Vakulenko N A, Levkovich N M
Antibiotiki. 1978 Aug;23(8):700-5.
The effect of various agar grades on the size and margin character of the inhibition growth zones in assay of antibiotic activity by the agar diffusion method was studied. It was shown that not all the agar grades could be used in antibiotic activity assay. Depending on the agar type the size of the inhibition growth zones produced by the same antibiotic concentration significantly varied. The variations in the size of the inhibition growth zones depended on the agar ability to bind antibiotics and were mainly defined by the agar purity. The agars with low content of nitrogen admixtures bound the antibiotics to a low extent. The commerical grades of the agars of the South-Sea and Korsakov Plants, the experimental grade of the TINRO agar with additional purification, as well as the agars imported from Argentina and France proved to be most useful for determination of the antibiotic activity by the agar diffusion method.
研究了不同等级琼脂在琼脂扩散法测定抗生素活性时对抑菌圈大小和边缘特征的影响。结果表明，并非所有等级的琼脂都可用于抗生素活性测定。取决于琼脂类型，相同抗生素浓度产生的抑菌圈大小显著不同。抑菌圈大小的变化取决于琼脂结合抗生素的能力，且主要由琼脂纯度决定。含氮杂质含量低的琼脂结合抗生素的程度较低。事实证明，南海植物和科尔萨科夫工厂生产的商业级琼脂、经过额外纯化的俄罗斯海洋渔业与海洋学研究所（TINRO）实验级琼脂，以及从阿根廷和法国进口的琼脂，最适用于琼脂扩散法测定抗生素活性。
