Minakawa H, Kitaura K
Jpn J Antibiot. 1975 Oct;28(5):669-71.
A simplified bioassay system for antibiotics has been presented. Glass tubes with open ends and pits are filled with agar medium containing test microbes by immersing vertically into the agar medium while it is warm. These agar tubes are inserted in test solutions and incubated. The growth-inhibitory zones appear in respective tubes due to upward diffusion of the antibiotic. The heights of such zones from the bottom of agar tubes are measured.
已提出一种用于抗生素的简化生物测定系统。将带有开口端和凹坑的玻璃管垂直浸入温热的琼脂培养基中,使其充满含有测试微生物的琼脂培养基。将这些琼脂管插入测试溶液中并进行培养。由于抗生素向上扩散,各个试管中会出现生长抑制区。测量这些区域从琼脂管底部起的高度。