Becker G L, Termine J D, Eanes E D
Calcif Tissue Res. 1976 Oct 12;21(2):105-13. doi: 10.1007/BF02547386.
Subcellular fractions of cytoplasmic mineral granules and mineral-loaded mitochondria were isolated from whole homogenates of hepatopancreas of the blue crab (Callinectes sapidus). Chemical, physical, and morphologic studies were carried out on both subcellular fractions, with and without prior removal of organic components by hydrazine extraction. In contrast to cytoplasmic granules, whole mitochondria contained appreciable amounts of mineral ions not associated with a solid mineral phase. Quantitative analyses and infrared spectroscopy showed cytoplasmic and mitochondrial mineral phases to be calcium phosphates of similar but not identical composition. Both cytoplasmic and mitochondrial mineral phases, as in synthetic amorphous calcium phosphate, show noncrystalline patterns when examined by infrared spectroscopy and x-ray diffraction, and a common ultrastructure of clustered spheres of approximately 100 A diameter. The findings suggest that amorphous calcium phosphate in biological systems may exhibit appreciable variation in Ca/P and in the content of foreign ions such as Mg2+, ADP, and ATP. A mitochondriogenic mechanism of calcification could not be confirmed nor refuted by this study.
从蓝蟹（Callinectes sapidus）肝胰腺的全匀浆中分离出细胞质矿物颗粒和载有矿物质的线粒体的亚细胞组分。对这两种亚细胞组分进行了化学、物理和形态学研究，包括在肼提取去除有机成分之前和之后。与细胞质颗粒不同，完整的线粒体含有大量与固体矿物相无关的矿物离子。定量分析和红外光谱显示细胞质和线粒体矿物相是组成相似但不完全相同的磷酸钙。细胞质和线粒体矿物相，如同合成无定形磷酸钙一样，在通过红外光谱和X射线衍射检查时显示非晶态模式，并且具有直径约100埃的聚集球体的共同超微结构。这些发现表明生物系统中的无定形磷酸钙在钙/磷以及诸如Mg2+、ADP和ATP等外来离子的含量方面可能表现出明显的变化。本研究既不能证实也不能反驳钙化的线粒体发生机制。
