Brockman R P, Manns J G, Bergman E N
Can J Physiol Pharmacol. 1976 Oct;54(5):666-70. doi: 10.1139/y76-092.
The secretion of immunoreactive glucagon (IRG) into the portal blood and its removal by the liver were determined in conscious-fed sheep by simultaneous measurement of venoarterial plasma concentration differences and portal and hepatic plasma flows. IRG was determined using Manns' antiserum and Unger's 30K antiserum, the latter being highly specific for pancreatic glucagon. In 21 experiments in which Manns' antiserum was used the IRG secretory rate was 7.1 +/- u.4 mug/h. The value using 30K antiserum was lower (5.5 +/- 1.3, n=6), but not significantly different. Although the hepatic extraction ratio (hepatic removal - total IRG presented to the liver) was only 7%, the hepatic removal of 2.4 +/- 0.5 mug/h was equivalent to 31-35% of the portal IRG secretory rate. Since during steady-state conditions, glucagon secretion equals glucagon removal, the liver must account for approximately one-third of the glucagon degraded by the entire body.
通过同时测量门静脉与动脉血浆浓度差以及门静脉和肝血浆流量，测定清醒进食绵羊门静脉血中免疫反应性胰高血糖素（IRG）的分泌及其被肝脏清除的情况。使用曼氏抗血清和昂格尔氏30K抗血清测定IRG，后者对胰高血糖素具有高度特异性。在使用曼氏抗血清的21次实验中，IRG分泌率为7.1±0.4微克/小时。使用30K抗血清的值较低（5.5±1.3，n = 6），但差异不显著。尽管肝脏提取率（肝脏清除 - 进入肝脏的总IRG）仅为7%，但肝脏每小时清除2.4±0.5微克相当于门静脉IRG分泌率的31 - 35%。由于在稳态条件下，胰高血糖素分泌等于胰高血糖素清除，肝脏必须占全身降解的胰高血糖素的约三分之一。
