Stoffel W, Sorgo W
Chem Phys Lipids. 1976 Oct;17(2-3 SPEC NO):324-35. doi: 10.1016/0009-3084(76)90077-3.
The organization of the lipid bilayer of the enveloped Sindbis virus has been studied. In the model membrane which consists only of two virus specific glycoproteins and host derived lipids the latter were radioactively labelled with 14C-palmitic acid by prelabelling their BHK 21 host cell lipids. The purified virus particles were submitted to neuramidase, bromelain and combromelain-neuraminidase treatment. It could be demonstrated that N-acetyl neuraminic acid residue of the total hematoside present in the virion is hydrolyzed by neuraminidase leaving the particles fully intact. Proteolysis of the spikes leads to particle aggregation yet an unchanged hematoside content. This was fully transformed into ceramidelactoside by subsequent neuraminidase treatment. The analyses of the ceramide species present in hematoside of the control particles and ceramidelactoside derived thereof by neuraminidase hydrolysis are in very close agreement. From these experiments it is concluded that all hematoside molecules are organized in the outer half of the bilayer of the envelope.
对有包膜的辛德毕斯病毒脂质双层的组织进行了研究。在仅由两种病毒特异性糖蛋白和宿主来源脂质组成的模型膜中，通过对其BHK 21宿主细胞脂质进行预标记，用14C-棕榈酸对后者进行放射性标记。将纯化的病毒颗粒进行神经氨酸酶、菠萝蛋白酶和菠萝蛋白酶-神经氨酸酶处理。可以证明，病毒体中存在的总血苷的N-乙酰神经氨酸残基被神经氨酸酶水解，而颗粒保持完全完整。刺突的蛋白水解导致颗粒聚集，但血苷含量不变。随后的神经氨酸酶处理将其完全转化为神经酰胺乳糖苷。通过神经氨酸酶水解对对照颗粒血苷中存在的神经酰胺种类及其衍生的神经酰胺乳糖苷进行分析，结果非常一致。从这些实验得出结论，所有血苷分子都排列在包膜双层的外半部分。
