Stoffel W, Anderson R, Stahl J
Hoppe Seylers Z Physiol Chem. 1975 Jul;356(7):1123-9. doi: 10.1515/bchm2.1975.356.2.1123.
Lipids of BHK 21 cells (baby hamster kidney) grown in tissue culture were labelled with radioactive fatty acids. The enveloped vesicular stomatitis virus was propagated in this host cell type. The virions were purified by density gradient centrifugation. Neuraminidase treatment of the intact virions led to a complete transformation of hematoside [N-acetylneuraminosyl(alpha2-3)lactosyl(beta1-1)ceramide] into lactosylceramide, with identical labelling of the ceramide portion in hematoside of the untreated virions and the lactosylceramide of the neuraminidase-treated particles. The morphology of the virions appeared unchanged in electron micrographs, but the neuraminic-acid-free virions had a strong tendency to aggregate. The results of these studies are evidence that gangliosides are integrated exclusively into the outer lamella of the lipid bilayer in the viral envelope. It is also evident that the viral envelope is a suitable model for studies on membrane asymmetry.
在组织培养中生长的BHK 21细胞（幼仓鼠肾细胞）的脂质用放射性脂肪酸进行标记。包膜水泡性口炎病毒在这种宿主细胞类型中繁殖。病毒粒子通过密度梯度离心法进行纯化。用神经氨酸酶处理完整的病毒粒子导致血型糖苷（N-乙酰神经氨酸基（α2-3）乳糖基（β1-1）神经酰胺）完全转化为乳糖基神经酰胺，未处理病毒粒子的血型糖苷中的神经酰胺部分与经神经氨酸酶处理的颗粒中的乳糖基神经酰胺具有相同的标记。在电子显微镜照片中，病毒粒子的形态似乎没有变化，但不含神经氨酸的病毒粒子有强烈的聚集倾向。这些研究结果证明神经节苷脂仅整合到病毒包膜脂质双层的外层薄片中。同样明显的是，病毒包膜是研究膜不对称性的合适模型。
