Simon A, Romert A, Gustafson A L, McCaffery J M, Eriksson U
Ludwig Institute for Cancer Research, Stockholm Branch, Box 240, S-171 77 Stockholm, Sweden.
J Cell Sci. 1999 Feb;112 ( Pt 4):549-58. doi: 10.1242/jcs.112.4.549.
11-cis retinol dehydrogenase (EC 1.1.1.105) catalyses the last step in the biosynthetic pathway generating 11-cis retinaldehyde, the common chromophore of all visual pigments in higher animals. The enzyme is abundantly expressed in retinal pigment epithelium of the eye and is a member of the short chain dehydrogenase/reductase superfamily. In this work we demonstrate that a majority of 11-cis retinol dehydrogenase is associated with the smooth ER in retinal pigment epithelial cells and that the enzyme is an integral membrane protein, anchored to membranes by two hydrophobic peptide segments. The catalytic domain of the enzyme is confined to a lumenal compartment and is not present on the cytosolic aspect of membranes. Thus, the subcellular localization and the membrane topology of 11-cis retinol dehydrogenase suggest that generation of 11-cis retinaldehyde is a compartmentalized process.
11-顺式视黄醇脱氢酶（EC 1.1.1.105）催化生物合成途径中的最后一步反应，生成11-顺式视黄醛，这是高等动物中所有视觉色素的共同发色团。该酶在眼睛的视网膜色素上皮中大量表达，是短链脱氢酶/还原酶超家族的成员。在这项研究中，我们证明，大多数11-顺式视黄醇脱氢酶与视网膜色素上皮细胞中的滑面内质网相关，并且该酶是一种整合膜蛋白，通过两个疏水肽段锚定在膜上。该酶的催化结构域局限于腔室，不存在于膜的胞质面。因此，11-顺式视黄醇脱氢酶的亚细胞定位和膜拓扑结构表明，11-顺式视黄醛的生成是一个区室化过程。
