Schwartz E B, Granger D A, Susman E J, Gunnar M R, Laird B
Pennslyvania State University, USA.
Child Dev. 1998 Dec;69(6):1503-13.
In a series of studies, we evaluated the susceptibility of radioimmunoassays (RIA) for saliva cortisol to interference effects caused by oral stimulants used to facilitate saliva collection in studies with children. When added directly to saliva samples, oral stimulants (drink mix crystals) artificially inflated estimated cortisol concentrations. The magnitude of the interference effect was concentration-dependent and more pronounced for some stimulants and RIA procedures than for others. Analysis of samples collected using oral stimulants from child and adult participants confirmed stimulant interference as an extraneous source of variability in measured saliva cortisol. Associations between serum and saliva cortisol and between saliva cortisol and "behavioral" variables were attenuated by stimulant interference. A survey of six large child studies estimated interference effects, indexed by low sample pH, to be present in 14.7% of the 1,148 total saliva samples, or 2%-54% (M = 22%) of samples within each study. Recommendations to minimize the impact of stimulant interference in studies involving salivary cortisol in the context of child health and development are outlined.
在一系列研究中，我们评估了用于儿童研究中促进唾液采集的口服兴奋剂对唾液皮质醇放射免疫分析（RIA）干扰效应的敏感性。当直接添加到唾液样本中时，口服兴奋剂（饮料混合晶体）会人为地提高估计的皮质醇浓度。干扰效应的大小取决于浓度，并且对于某些兴奋剂和RIA程序而言比其他的更明显。对使用口服兴奋剂从儿童和成人参与者收集的样本进行分析证实，兴奋剂干扰是测量唾液皮质醇中变异性的一个额外来源。血清与唾液皮质醇之间以及唾液皮质醇与“行为”变量之间的关联因兴奋剂干扰而减弱。对六项大型儿童研究的调查估计，以低样本pH值为指标的干扰效应存在于1148个总唾液样本中的14.7%，或每项研究中样本的2%-54%（中位数 = 22%）。本文概述了在儿童健康与发育背景下尽量减少兴奋剂干扰对涉及唾液皮质醇研究影响的建议。
