Jiang X R, Jimenez G, Chang E, Frolkis M, Kusler B, Sage M, Beeche M, Bodnar A G, Wahl G M, Tlsty T D, Chiu C P
Geron Corporation, Menlo Park, California 94025, USA.
Nat Genet. 1999 Jan;21(1):111-4. doi: 10.1038/5056.
Expression of the human telomerase catalytic component, hTERT, in normal human somatic cells can reconstitute telomerase activity and extend their replicative lifespan. We report here that at twice the normal number of population doublings, telomerase-expressing human skin fibroblasts (BJ-hTERT) and retinal pigment epithelial cells (RPE-hTERT) retain normal growth control in response to serum deprivation, high cell density, G1 or G2 phase blockers and spindle inhibitors. In addition, we observed no cell growth in soft agar and detected no tumour formation in vivo. Thus, we find that telomerase expression in normal cells does not appear to induce changes associated with a malignant phenotype.
人类端粒酶催化亚基hTERT在正常人体体细胞中的表达可重建端粒酶活性并延长其复制寿命。我们在此报告，在群体倍增次数达到正常两倍时，表达端粒酶的人皮肤成纤维细胞（BJ-hTERT）和视网膜色素上皮细胞（RPE-hTERT）在血清剥夺、高细胞密度、G1或G2期阻滞剂以及纺锤体抑制剂作用下仍保持正常生长控制。此外，我们在软琼脂中未观察到细胞生长，在体内也未检测到肿瘤形成。因此，我们发现正常细胞中的端粒酶表达似乎不会诱导与恶性表型相关的变化。
