巢蛋白基因增强子揭示神经祖细胞的异质性
Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene.
作者信息
Yaworsky P J, Kappen C
机构信息
Molecular Neuroscience Program, Samuel C. Johnson Medical Research Center, Scottsdale, Arizona, 85259, USA.
出版信息
Dev Biol. 1999 Jan 15;205(2):309-21. doi: 10.1006/dbio.1998.9035.
Abstract
Using transgenic embryos, we have identified two distinct CNS progenitor cell-specific enhancers, each requiring the cooperation of at least two independent regulatory sites, within the second intron of the rat nestin gene. One enhancer is active throughout the developing CNS, while the other is specifically active in the ventral midbrain. These experiments demonstrate that neural progenitor cells in the midbrain constitute a unique subpopulation based upon their ability to activate the midbrain regulatory element. Our finding of differential enhancer activity from a gene encoding a structural protein reveals a previously unrecognized diversity in neural progenitor cell populations.
摘要
利用转基因胚胎，我们在大鼠巢蛋白基因的第二个内含子中鉴定出两个不同的中枢神经系统祖细胞特异性增强子，每个增强子都需要至少两个独立调控位点的协同作用。其中一个增强子在整个发育中的中枢神经系统中都有活性，而另一个则在腹侧中脑特异性地活跃。这些实验表明，中脑中的神经祖细胞基于其激活中脑调控元件的能力构成了一个独特的亚群。我们从一个编码结构蛋白的基因中发现增强子活性存在差异，这揭示了神经祖细胞群体中一种以前未被认识到的多样性。
相似文献
1
Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene.巢蛋白基因增强子揭示神经祖细胞的异质性
Dev Biol. 1999 Jan 15;205(2):309-21. doi: 10.1006/dbio.1998.9035.
2
An evolutionarily conserved region in the second intron of the human nestin gene directs gene expression to CNS progenitor cells and to early neural crest cells.人类巢蛋白基因第二个内含子中的一个进化保守区域将基因表达导向中枢神经系统祖细胞和早期神经嵴细胞。
Eur J Neurosci. 1997 Mar;9(3):452-62. doi: 10.1111/j.1460-9568.1997.tb01622.x.
3
Identification of both general and region-specific embryonic CNS enhancer elements in the nestin promoter.在巢蛋白启动子中鉴定一般和区域特异性胚胎中枢神经系统增强子元件。
Exp Cell Res. 1999 May 1;248(2):509-19. doi: 10.1006/excr.1999.4417.
4
Mutation of a putative nuclear receptor binding site abolishes activity of the nestin midbrain enhancer.
Biochim Biophys Acta. 2003 Jan 3;1625(1):109-15. doi: 10.1016/s0167-4781(02)00588-2.
5
Nestin enhancer requirements for expression in normal and injured adult CNS.巢蛋白增强子在正常和损伤的成年中枢神经系统中表达的需求
J Neurosci Res. 2002 Sep 15;69(6):784-94. doi: 10.1002/jnr.10376.
6
Independent regulatory elements in the nestin gene direct transgene expression to neural stem cells or muscle precursors.巢蛋白基因中的独立调控元件将转基因表达导向神经干细胞或肌肉前体细胞。
Neuron. 1994 Jan;12(1):11-24. doi: 10.1016/0896-6273(94)90148-1.
7
POU transcription factors control expression of CNS stem cell-specific genes.POU转录因子控制中枢神经系统干细胞特异性基因的表达。
Development. 1998 Aug;125(16):3087-100. doi: 10.1242/dev.125.16.3087.
8
Transgenic analysis of central nervous system development and regeneration.中枢神经系统发育与再生的转基因分析
Acta Anaesthesiol Scand Suppl. 1997;110:116-8. doi: 10.1111/j.1399-6576.1997.tb05524.x.
9
Different regions of the mouse nestin enhancer may function differentially in nestin expression in an NSC-like cell line and astrocytes.小鼠巢蛋白增强子的不同区域可能在类神经干细胞系和星形胶质细胞的巢蛋白表达中发挥不同作用。
Neurosci Lett. 2005 May 6;379(2):90-5. doi: 10.1016/j.neulet.2004.12.048.
10
Nestin-EGFP transgenic mice: visualization of the self-renewal and multipotency of CNS stem cells.巢蛋白-增强绿色荧光蛋白转基因小鼠：中枢神经系统干细胞自我更新和多能性的可视化
Mol Cell Neurosci. 2001 Feb;17(2):259-73. doi: 10.1006/mcne.2000.0925.
引用本文的文献
1
Stable knockdown of Drp1 improves retinoic acid-BDNF-induced neuronal differentiation through global transcriptomic changes and results in reduced phosphorylation of ERK1/2 independently of DUSP1 and 6.Drp1的稳定敲低通过整体转录组变化改善视黄酸-BDNF诱导的神经元分化，并导致ERK1/2的磷酸化减少，且与DUSP1和6无关。
Front Cell Dev Biol. 2024 Mar 14;12:1342741. doi: 10.3389/fcell.2024.1342741. eCollection 2024.
2
Identification of a Chondrocyte-Specific Enhancer in the Gene.在该基因中鉴定软骨细胞特异性增强子。
J Dev Biol. 2024 Jan 24;12(1):5. doi: 10.3390/jdb12010005.
3
Use of Brain-Derived Stem/Progenitor Cells and Derived Extracellular Vesicles to Repair Damaged Neural Tissues: Lessons Learned from Connective Tissue Repair Regarding Variables Limiting Progress and Approaches to Overcome Limitations.利用脑源性干细胞/祖细胞及其衍生的细胞外囊泡修复受损的神经组织：结缔组织修复中关于限制进展的变量和克服限制的方法所得到的启示。
Int J Mol Sci. 2023 Feb 8;24(4):3370. doi: 10.3390/ijms24043370.
4
Structural variation during dog domestication: insights from gray wolf and dhole genomes.犬驯化过程中的结构变异：来自灰狼和亚洲豺犬基因组的见解
Natl Sci Rev. 2019 Jan;6(1):110-122. doi: 10.1093/nsr/nwy076. Epub 2018 Jul 19.
5
Circulating Nestin-GFP Cells Participate in the Pathogenesis of Paracoccidioides brasiliensis in the Lungs.循环巢蛋白-GFP 细胞参与巴西副球孢子菌在肺部的发病机制。
Stem Cell Rev Rep. 2021 Oct;17(5):1874-1888. doi: 10.1007/s12015-021-10181-3. Epub 2021 May 18.
6
MiRNA Regulatory Functions in Photoreceptors.微小RNA在光感受器中的调控功能
Front Cell Dev Biol. 2021 Jan 21;8:620249. doi: 10.3389/fcell.2020.620249. eCollection 2020.
7
Craniofacial skeletal response to encephalization: How do we know what we think we know?颅面骨骼对脑化的反应：我们如何知道我们自认为知道的事情？
Am J Phys Anthropol. 2019 Jan;168 Suppl 67(Suppl 67):27-46. doi: 10.1002/ajpa.23766.
8
Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis.联合实验和系统水平分析揭示了 miR-124 在人类神经发生过程中的复杂调控网络。
Cell Syst. 2018 Oct 24;7(4):438-452.e8. doi: 10.1016/j.cels.2018.08.011. Epub 2018 Oct 3.
9
The Biological Role of Nestin-Cells in Physiological and Pathological Cardiovascular Remodeling.巢蛋白阳性细胞在生理性和病理性心血管重塑中的生物学作用
Front Cell Dev Biol. 2018 Feb 14;6:15. doi: 10.3389/fcell.2018.00015. eCollection 2018.
10
HMGB1/Advanced Glycation End Products (RAGE) does not aggravate inflammation but promote endogenous neural stem cells differentiation in spinal cord injury.高迁移率族蛋白 B1/晚期糖基化终产物（RAGE）不会加重炎症反应，反而促进脊髓损伤内源性神经干细胞的分化。
Sci Rep. 2017 Sep 4;7(1):10332. doi: 10.1038/s41598-017-10611-8.
本文引用的文献
1
Transgenic analyses reveal developmentally regulated neuron- and muscle-specific elements in the murine neurofilament light chain gene promoter.转基因分析揭示了小鼠神经丝轻链基因启动子中受发育调控的神经元和肌肉特异性元件。
J Biol Chem. 1997 Oct 3;272(40):25112-20. doi: 10.1074/jbc.272.40.25112.
2
Limb proprioceptive deficits without neuronal loss in transgenic mice overexpressing neurotrophin-3 in the developing nervous system.在发育中的神经系统中过表达神经营养因子-3的转基因小鼠出现肢体本体感觉缺陷但无神经元丢失。
Development. 1997 Jul;124(13):2603-13. doi: 10.1242/dev.124.13.2603.
3
The intermediate filament protein nestin occurs transiently in differentiating testis of rat and mouse.中间丝蛋白巢蛋白在大鼠和小鼠的分化睾丸中短暂出现。
Differentiation. 1997 May;61(4):243-9. doi: 10.1046/j.1432-0436.1997.6140243.x.
4
An evolutionarily conserved region in the second intron of the human nestin gene directs gene expression to CNS progenitor cells and to early neural crest cells.人类巢蛋白基因第二个内含子中的一个进化保守区域将基因表达导向中枢神经系统祖细胞和早期神经嵴细胞。
Eur J Neurosci. 1997 Mar;9(3):452-62. doi: 10.1111/j.1460-9568.1997.tb01622.x.
5
Dopamine neuron agenesis in Nurr1-deficient mice.Nurr1基因缺陷小鼠中的多巴胺神经元发育不全
Science. 1997 Apr 11;276(5310):248-50. doi: 10.1126/science.276.5310.248.
6
Stem cells in the central nervous system.中枢神经系统中的干细胞。
Science. 1997 Apr 4;276(5309):66-71. doi: 10.1126/science.276.5309.66.
7
Regulatory mechanisms in stem cell biology.干细胞生物学中的调控机制。
Cell. 1997 Feb 7;88(3):287-98. doi: 10.1016/s0092-8674(00)81867-x.
8
Neural stem cells are blasting off.神经干细胞正在蓬勃发展。
Neuron. 1997 Jan;18(1):1-4. doi: 10.1016/s0896-6273(01)80018-0.
9
neurogenins, a novel family of atonal-related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS.神经发生素是与无调蛋白相关的bHLH转录因子的一个新家族，是假定的哺乳动物神经元决定基因，可揭示发育中的中枢神经系统和外周神经系统中祖细胞的异质性。
Mol Cell Neurosci. 1996;8(4):221-41. doi: 10.1006/mcne.1996.0060.
10
Expression of the Notch 3 intracellular domain in mouse central nervous system progenitor cells is lethal and leads to disturbed neural tube development.Notch 3细胞内结构域在小鼠中枢神经系统祖细胞中的表达是致死性的，并导致神经管发育紊乱。
Mech Dev. 1996 Oct;59(2):177-90. doi: 10.1016/0925-4773(96)00589-8.
Zotero 插件