Non-linear microscale alterations in membrane transport by electropermeabilization.

The purpose of this study was to quantify the changes in cell membrane conductance in response to electropermeabilization, which may elucidate the mechanisms of tissue injury resulting from high-voltage electrical shock. A high-speed, space-clamp and voltage-clamp experimental configuration was used. The pulse parameters of an imposed transmembrane potential that are instrumental in membrane properties alteration were precisely controlled. The dynamics of the non-linear electroporation response was characterized.