Uehara M, Tateishi S, Chiba H, Suzuki K, Goto S
Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, Japan.
J Nutr Sci Vitaminol (Tokyo). 1998 Oct;44(5):705-14. doi: 10.3177/jnsv.44.705.
Free radicals and lipid peroxide formation are related to tissue damage. This damage is thought to be associated with various diseases. To accurately assess the degree of lipid hydroperoxidation in biological materials, three different thiobarbituric acid (TBA) assay methods and the measurement of phosphatidylcholine hydroperoxide (PCOOH) by the chemiluminescence-HPLC (CL-HPLC) method were compared. Iron and copper deficiencies in rats reduced the contents of these metals. The addition of iron and copper to liver homogenates produced dose-dependent increases and decreases in TBARS, respectively. TBARS measured by the Shinnhuber method slightly increased with the addition of copper. The measurement of PCOOH was less affected by the addition of these metals. TBARS did not reflect lipid peroxidation when different concentrations of metals were present in the samples. The TBARS level is thus not a suitable marker for the assess of lipid peroxidation. PCOOH, accumulated as a primary peroxidation product from membrane phospholipids in the liver, was found to be a more appropriate marker for the estimating hepatic lipid peroxidation in the iron- and/or copper-deficient condition.
自由基和脂质过氧化物的形成与组织损伤有关。这种损伤被认为与多种疾病相关。为了准确评估生物材料中脂质过氧化的程度，比较了三种不同的硫代巴比妥酸（TBA）检测方法以及通过化学发光-高效液相色谱（CL-HPLC）法测定磷脂酰胆碱氢过氧化物（PCOOH）。大鼠缺铁和缺铜会降低这些金属的含量。向肝匀浆中添加铁和铜分别使硫代巴比妥酸反应物（TBARS）含量呈剂量依赖性增加和减少。用Shinnhuber方法测定的TBARS随铜的添加略有增加。添加这些金属对PCOOH的测定影响较小。当样品中存在不同浓度的金属时，TBARS不能反映脂质过氧化。因此，TBARS水平不是评估脂质过氧化的合适标志物。PCOOH作为肝脏中膜磷脂的主要过氧化产物积累，被发现是评估缺铁和/或缺铜条件下肝脏脂质过氧化的更合适标志物。
