High-resolution three-dimensional magnetic resonance imaging for the investigation of knee joint damage during the time course of antigen-induced arthritis in rabbits.

OBJECTIVE

To investigate the efficacy of high-resolution 3-dimensional magnetic resonance imaging (3-D MRI) with gadolinium contrast agent enhancement in monitoring soft tissue and hard tissue changes during the course of antigen-induced arthritis in rabbits, comparing the images with the final histologic outcome.

METHODS

Arthritis was induced by intraarticular injection of methylated bovine serum albumin into rabbits that had been presensitized to the same antigen. MRI was carried out before and on predetermined days after the onset of inflammatory arthritis, up to day 60. At each time point, 3-D gradient-echo images were acquired. At the end of the study, the knee joints were processed for histologic study.

RESULTS

Time-related inflammatory changes, such as soft tissue swelling, were seen initially, followed by a reduction in the acute swelling and a progression to hard tissue damage at later time points. The final 3-D data sets were then used to compare MR images with the histologic sections. The knee joints were sectioned in the sagittal, coronal, or transverse direction. Comparison of final images and histologic features at day 60 enabled a more complete interpretation of the MR images, allowing direct correlation of joint damage observed using the MRI technique with that seen in the final histologic analysis.

CONCLUSION

Qualitative information derived from MR images correlated extremely well with histologic findings. These results indicate the great potential of MRI for noninvasive investigation of drug effects in this model of arthritis.