Entrala C, Lorente M, Lorente J A, Alvarez J C, Moretti T, Budowle B, Villanueva E
Dept. de Medicina Legal, Facultad de Medicina, Universidad de Granada, Spain.
Forensic Sci Int. 1998 Dec 21;98(3):179-83. doi: 10.1016/s0379-0738(98)00165-0.
A total of 171 Caucasians living in Andalucia (southern Spain) have been typed for nine short tandem repeat (STR) loci by multiplex PCR amplification using a commercially available kit (Profiler Plus; Perkin-Elmer, Norwalk, CT, USA) and semi-automatic electrophoresis (ABI Prism 377 DNA Sequencer, Applied Biosystems, Foster City, CA, USA). The kit enables typing of the STR loci D3S1358, VWA, FGA, D5S818, D13S317, D7S820, D8S1179, D21S11, and D18S51. All loci, except D7S820, meet Hardy-Weinberg equilibrium. Because of the large number of loci that can be analyzed, the power of discrimination (PD) is greater than 0.99999, and the probability of exclusion (PE) reaches 0.99991 in our population sample.
共有171名居住在安达卢西亚（西班牙南部）的高加索人，使用市售试剂盒（Profiler Plus；美国康涅狄格州诺沃克市珀金埃尔默公司）通过多重PCR扩增和半自动电泳（美国加利福尼亚州福斯特城应用生物系统公司的ABI Prism 377 DNA测序仪）对9个短串联重复序列（STR）基因座进行了分型。该试剂盒能够对STR基因座D3S1358、VWA、FGA、D5S818、D13S317、D7S820、D8S1179、D21S11和D18S51进行分型。除D7S820外，所有基因座均符合哈迪-温伯格平衡。由于可分析的基因座数量众多，在我们的人群样本中，鉴别力（PD）大于0.99999，排除概率（PE）达到0.99991。
