Bykowski T, Sirko A
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
Biochimie. 1998 Dec;80(12):987-1001. doi: 10.1016/s0300-9084(99)80004-2.
In attempts to identify subunit-specific phenotypes of ihf mutants we analyzed viability, thermoresistance and protein synthesis patterns in ihfA and ihfB mutants and their respective parental strains. Despite some detected differences in the two-dimensional protein patterns, no significant subunit-specific, physiological effects could be observed. Each mutant was less viable and less thermoresistant than the wild type strain. Moreover, in contrast to the wild type the mutants did not reduce global protein synthesis after prolonged culturing. Examination of expression of transcriptional fusions allowed us to demonstrate autoregulation of both genes by IHF. Additional IHF binding sites in the regulatory region of both ihf genes were footprinted.
为了确定整合宿主因子（IHF）突变体的亚基特异性表型，我们分析了ihfA和ihfB突变体及其各自亲本菌株的活力、耐热性和蛋白质合成模式。尽管在二维蛋白质图谱中检测到了一些差异，但未观察到显著的亚基特异性生理效应。每个突变体的活力和耐热性均低于野生型菌株。此外，与野生型不同，突变体在长时间培养后并未降低整体蛋白质合成。对转录融合表达的检测使我们能够证明IHF对这两个基因的自动调节作用。在两个ihf基因的调控区域中发现了额外的IHF结合位点。
