Strnadová K
Folia Microbiol (Praha). 1976;21(6):455-8. doi: 10.1007/BF02876936.
A simple and speedy procedure is described for the preparation and application of nitrous acid in mutagenic experiments. The conventional incubation mixture of sodium nitrite solution with acetate buffer is replaced by an aqueous solution of nitrous acid prepared by running a solution of sodium nitrite through a column of ion exchange. The procedure eliminates also the interference of other substances. Experimental appraisal of the technique was done during the mutagenic improval of the fungus Claviceps purpurea.
本文描述了一种在诱变实验中制备和应用亚硝酸的简单快速方法。用亚硝酸钠溶液通过离子交换柱制备的亚硝酸水溶液代替了传统的亚硝酸钠溶液与醋酸盐缓冲液的孵育混合物。该方法还消除了其他物质的干扰。在对真菌麦角菌进行诱变改良的过程中对该技术进行了实验评估。
