对日本分离出的335株铜绿假单胞菌进行gyrA基因突变检测及其对氟喹诺酮类药物的敏感性分析。
Detection of gyrA mutations among 335 Pseudomonas aeruginosa strains isolated in Japan and their susceptibilities to fluoroquinolones.
作者信息
Takenouchi T, Sakagawa E, Sugawara M
机构信息
Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
出版信息
Antimicrob Agents Chemother. 1999 Feb;43(2):406-9. doi: 10.1128/AAC.43.2.406.
Abstract
gyrA point mutations in 335 clinical Pseudomonas aeruginosa isolates were examined mainly by nonisotopic single-strand conformation polymorphism analysis and direct sequencing. Seven types of missense gyrA mutations were observed in 70 of 335 strains (20.9%), and ciprofloxacin MICs were > or = 3.13 micrograms/ml for 63 of 70 strains (90.0%). These included two double point mutations and three novel mutations (Ala-67-->Ser plus Asp-87-->Gly, Ala-84-->Pro, and Gln-106-->Leu). Thr-83-->Ile mutants were predominantly observed (63 of 70 mutants) and showed high-level fluoroquinolone resistance (ciprofloxacin MIC at which 50% of isolates are inhibited, 25 micrograms/ml).
摘要
主要通过非同位素单链构象多态性分析和直接测序检测了335株临床铜绿假单胞菌分离株中的gyrA点突变。在335株菌株中的70株（20.9%）观察到7种错义gyrA突变，70株中的63株（90.0%）环丙沙星的最低抑菌浓度（MIC）≥3.13微克/毫升。这些突变包括两个双点突变和三个新突变（丙氨酸-67→丝氨酸加天冬氨酸-87→甘氨酸、丙氨酸-84→脯氨酸和谷氨酰胺-106→亮氨酸）。苏氨酸-83→异亮氨酸突变体占主导（70个突变体中的63个），并表现出高水平的氟喹诺酮耐药性（50%分离株被抑制时的环丙沙星MIC为25微克/毫升）。
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