Smith O P, Durkin S A, Luster D G, McDaniel L L, Russo A J, Damsteegt V D
Department of Biology, Hood College, Frederick, MD 21701, USA.
Virus Genes. 1998;17(3):207-11. doi: 10.1023/a:1008012219123.
The nucleotide sequence of the coat protein gene of the dwarfing (D) strain of soybean dwarf luteovirus (SbDV) was determined from cloned cDNA. The gene contains 600 nucleotides and encodes a protein of 200 amino acids with a calculated molecular mass of 22.2 kDa. A major portion of the coat protein open reading frame (ORF) was expressed in Escherichia coli as a pET fusion protein and the product was detected by western blot analysis using SbDV-D polyclonal antibodies. Comparison of the deduced coat protein amino acid sequence to that from the yellowing (Y) strain of SbDV demonstrated 88% identity.
从克隆的cDNA中测定了大豆矮缩黄症病毒（SbDV）矮化（D）株系外壳蛋白基因的核苷酸序列。该基因含有600个核苷酸，编码一个由200个氨基酸组成的蛋白质，计算分子量为22.2 kDa。外壳蛋白开放阅读框（ORF）的主要部分在大肠杆菌中作为pET融合蛋白表达，产物通过使用SbDV-D多克隆抗体的蛋白质免疫印迹分析进行检测。将推导的外壳蛋白氨基酸序列与SbDV黄化（Y）株系的序列进行比较，显示出88%的同一性。
