Wong M L, Liu J J, Huang C, Chen J W, Chang T J
Graduate Institute of Veterinary Microbiology, National Chung Hsing University Taichung, Taiwan.
Virus Genes. 1998;17(3):213-8. doi: 10.1023/a:1008061603194.
A cDNA of the 3'-terminus of classical swine fever virus (LPC vaccine strain) was cloned and sequenced. The 3431 nucleotides and deduced amino acid sequences were compared with those of other pestiviruses, and the similarity of nucleotide sequences and deduced amino acid sequences were found to be 84-95% and 95-98%, respectively. Similar to other isolates of classical swine fever virus, the sequenced region included the non-structural gene p58 (NS5A) and part of p76 (NS5B) gene. The p76 gene of LPC vaccine strain also contained a highly conserved motif G-D-D (Gly-Asp-Asp) that is present in the RNA replicase of positive-stranded RNA viruses. With the sequence data currently available, we carried out a phylogenetic analysis and obtained a genealogical relationship among members of the classical swine fever virus.
克隆并测序了经典猪瘟病毒（LPC疫苗株）3'-末端的cDNA。将3431个核苷酸和推导的氨基酸序列与其他瘟病毒的序列进行了比较，发现核苷酸序列和推导氨基酸序列的相似性分别为84%-95%和95%-98%。与经典猪瘟病毒的其他分离株相似，测序区域包括非结构基因p58（NS5A）和部分p76（NS5B）基因。LPC疫苗株的p76基因还包含一个高度保守的基序G-D-D（甘氨酸-天冬氨酸-天冬氨酸），该基序存在于正链RNA病毒的RNA复制酶中。利用目前可用的序列数据，我们进行了系统发育分析，并获得了经典猪瘟病毒成员之间的谱系关系。
