果蝇神经胶质细胞缺失（gcm）基因的一种新型人类同源基因的分离与表达分析。
Isolation and expression analysis of a novel human homologue of the Drosophila glial cells missing (gcm) gene.
作者信息
Kanemura Y, Hiraga S, Arita N, Ohnishi T, Izumoto S, Mori K, Matsumura H, Yamasaki M, Fushiki S, Yoshimine T
机构信息
Department of Neurosurgery, Osaka University Medical School, Suita, Japan.
出版信息
FEBS Lett. 1999 Jan 15;442(2-3):151-6. doi: 10.1016/s0014-5793(98)01650-0.
A novel human homologue (GCMB) of the Drosophila glial cells missing gene (dGCM) was isolated using RACE. GCMB contained a gcm motif sequence and a nuclear targeting sequence similar to that of dGCM and mouse GCMb. Homology searches indicated that GCMB was located within chromosome 6p24.2. Transcripts of GCMB were detected by means of RT-PCR in fetal brain, normal adult kidney, 3/3 medulloblastomas, 1/3 gliomas and 4/8 non-neuroepithelial tumor cell lines. Our data suggest that humans have two homologues of gcm like mice and that human gcm genes form a novel family which may function not only during fetal development but also in the postnatal or pathological stage.
利用RACE技术分离出了果蝇神经胶质细胞缺失基因（dGCM）的一种新型人类同源物（GCMB）。GCMB包含一个gcm基序序列和一个与dGCM及小鼠GCMb相似的核定位序列。同源性搜索表明GCMB定位于6号染色体p24.2区域。通过RT-PCR在胎儿脑、正常成人肾脏、3/3的髓母细胞瘤、1/3的神经胶质瘤以及4/8的非神经上皮肿瘤细胞系中检测到了GCMB的转录本。我们的数据表明，人类和小鼠一样有两个gcm同源物，并且人类gcm基因形成了一个新的家族，其可能不仅在胎儿发育过程中发挥作用，还在出生后或病理阶段发挥作用。
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