Suzuki Y
Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Japan.
Ann N Y Acad Sci. 1998 Nov 30;855:252-4. doi: 10.1111/j.1749-6632.1998.tb10577.x.
The cytotoxic agent colchicine induced apoptotic cell death in the mouse olfactory epithelium and vomeronasal organ. The terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) method revealed the presence of many apoptotic bodies in the middle to basal region of the septal olfactory epithelium and vomeronasal organ near the boundary of the respiratory epithelium at 1 day after intraperitoneal (i.p.) injection of colchicine (4 mg/kg). In some areas of the nasal turbinates, massive apoptosis was observed in the olfactory epithelium. Electron micrographs showed that immature olfactory cells and globose basal cells were killed by the colchicine and had been phagocytized by the supporting cells and macrophages. In some areas of the nasal turbinates, mature olfactory cells were also killed, and thus only the supporting cells and horizontal basal cells remained in the epithelium. Bromodeoxyuridine (BrdU) labeling showed that regeneration occurred in the septum and vomeronasal organ at 4-6 days after colchicine treatment; however, there were no regenerated olfactory cells in some areas of the turbinates up to 30 days after colchicine treatment.
细胞毒性药物秋水仙碱可诱导小鼠嗅觉上皮和犁鼻器发生凋亡性细胞死亡。腹腔注射（i.p.）秋水仙碱（4mg/kg）1天后，末端脱氧核苷酸转移酶介导的生物素化脱氧尿苷三磷酸缺口末端标记（TUNEL）法显示，在鼻中隔嗅觉上皮和犁鼻器靠近呼吸上皮边界的中基区域存在许多凋亡小体。在鼻甲的一些区域，嗅觉上皮中观察到大量凋亡。电子显微镜照片显示，未成熟的嗅觉细胞和球状基底细胞被秋水仙碱杀死，并已被支持细胞和巨噬细胞吞噬。在鼻甲的一些区域，成熟的嗅觉细胞也被杀死，因此上皮中仅残留支持细胞和水平基底细胞。溴脱氧尿苷（BrdU）标记显示，秋水仙碱处理后4 - 6天，鼻中隔和犁鼻器发生再生；然而，秋水仙碱处理后30天，鼻甲的一些区域仍未出现再生的嗅觉细胞。
