Proshina O V, Chiriaeva O G, Smirnov A F
All-Russia Research Institute of Animal Genetics and Breeding, Russian Academy of Agricultural Sciences, Pushkin, St. Petersburg, Russia.
Genetika. 1998 Oct;34(10):1405-10.
Distribution of T-bands on mitotic chromosomes of Bos taurus was studied. Association of T-bands with telomeres and enrichment of T-bands with genes, with a known localization is described. After THA-banding on the chromosomes of cattle, telomeric and pericentromeric regions of all autosomes showed bright fluorescence. The exception was for chromosome 7, which did not have telomeric T-bands. Interstitial T-bands were detected only on chromosomes 7, 16, and Y (7q13, 7q15, 7q22, 7q24, 16q21, and Yp12). A total proportion of centromeric, telomeric, and interstitial T-bands was 11.19, 9.97, and 2.02% of the length of the haploid chromosome set, respectively. By means of fluorescent in situ hybridization (FISH), the presence of the telomeric repeat (TTAGGG)n was shown not only in telomeric regions of all autosome, but also in all pericentromeric regions. The obtained data are indicative of the specificity of T-banding on the chromosomes of Bos taurus.
研究了金牛座有丝分裂染色体上T带的分布情况。描述了T带与端粒的关联以及T带在已知定位基因中的富集情况。在牛染色体上进行THA显带后,所有常染色体的端粒和着丝粒区域均显示出明亮的荧光。例外的是7号染色体,它没有端粒T带。仅在7号、16号和Y染色体(7q13、7q15、7q22、7q24、16q21和Yp12)上检测到了中间T带。着丝粒、端粒和中间T带的总比例分别占单倍体染色体组长度的11.19%、9.97%和2.02%。通过荧光原位杂交(FISH)表明,端粒重复序列(TTAGGG)n不仅存在于所有常染色体的端粒区域,也存在于所有着丝粒区域。所获得的数据表明了金牛座染色体上T带显带的特异性。