Soukup J, Kodet R, Dahbiová R, Trka J, Zuna J
Ustav patologické anatomie 2. LF UK a FN Motol, Praha.
Cesk Patol. 1998 Nov;34(4):131-5.
We studied monoclonality of tumour cells by polymerase chain reaction (PCR) in paediatric lymphomas. In B-lymphomas we detected monoclonal rearrangement of the immunoglobulin of the T-cell receptor delta and gamma chain (TCR, TCR-delta, TCR-gamma) by an analogous way. We examined a group of 37 paediatric patients with lymphomas (26 with B-lymphomas and 11 with T-lymphomas). Monoclonal gene rearrangement was found in 34 cases, i.e. the method used in this study proved to be successful in 92% of cases. We confirmed our results by correlation with histologic and immunohistologic diagnoses. The method may be used as a complementary diagnostic tool in differential diagnosis of lymphoma versus non-neoplastic proliferation of lymphatic tissue, in distinction between B- and T-lymphomas, and to separate lymphomas and other malignancies. Beside the pure diagnostic usage we perceive the main importance of the method in monitoring minimal residual disease (MRD), because amplified products of PCR may be sequenced and used for preparing tumour specific primers and probes.
我们通过聚合酶链反应（PCR）研究了小儿淋巴瘤中肿瘤细胞的单克隆性。在B淋巴瘤中，我们以类似的方式检测到T细胞受体δ链和γ链（TCR、TCR-δ、TCR-γ）免疫球蛋白的单克隆重排。我们检查了一组37例小儿淋巴瘤患者（26例B淋巴瘤和11例T淋巴瘤）。在34例病例中发现了单克隆基因重排，即本研究中使用的方法在92%的病例中被证明是成功的。我们通过与组织学和免疫组织学诊断结果相关联来证实我们的结果。该方法可作为淋巴瘤与淋巴组织非肿瘤性增殖鉴别诊断、B淋巴瘤与T淋巴瘤区分以及淋巴瘤与其他恶性肿瘤区分的辅助诊断工具。除了单纯的诊断用途外，我们还认识到该方法在监测微小残留病（MRD）方面的主要重要性，因为PCR的扩增产物可以进行测序，并用于制备肿瘤特异性引物和探针。
