Rezuke W N, Abernathy E C, Tsongalis G J
Department of Pathology and Laboratory Medicine, Hartford Hospital, CT 06102, USA.
Clin Chem. 1997 Oct;43(10):1814-23.
Molecular diagnostic assays have become routine in the evaluation of lymphoid malignancies. Both Southern transfer and polymerase chain reaction (PCR) technologies are used to assess for B- and T-cell clonality, the presence of rearrangements involving protooncogenes such as bcl-1 and bcl-2, and the monitoring of minimal residual disease. We review the fundamentals of B- and T-cell ontogeny as well as the basic principles of the Southern transfer and PCR assays and their applications to the diagnosis of lymphoid malignancies.
分子诊断检测已成为评估淋巴系统恶性肿瘤的常规手段。Southern印迹法和聚合酶链反应（PCR）技术都用于评估B细胞和T细胞的克隆性、涉及原癌基因（如bcl-1和bcl-2）的重排情况以及微小残留病的监测。我们综述了B细胞和T细胞个体发生的基本原理，以及Southern印迹法和PCR检测的基本原理及其在淋巴系统恶性肿瘤诊断中的应用。
