Hegele R A, Sun F, Harris S B, Anderson C, Hanley A J, Zinman B
Blackburn Cardiovascular Genetics Laboratory, John P. Robarts Research Institute, London, Ontario, Canada.
J Hum Genet. 1999;44(1):10-4. doi: 10.1007/s100380050097.
We undertook a genome-wide scan using 190 markers with an average separation of 20 cM in 49 Canadian Oji-Cree sib pairs affected with type 2 diabetes. Four of these markers, one each on chromosomes 6, 8, 16, and 22, showed both suggestive linkage and suggestive association with type 2 diabetes in the Oji-Cree. None of these markers corresponded to any chromosomal region or marker that has so far been linked with type 2 diabetes in other populations. Thus, there might be several genetic loci that confer susceptibility to type 2 diabetes in this study sample. We are following up on these preliminary leads by increasing the density of the markers within these linked and associated regions, and also by increasing the number of study subjects. Also, we found instances in which there were wide disparities between the Oji-Cree and reference Caucasians with respect to marker heterozygosity. This suggests that a particular set of markers for genome-wide scanning will have different informativeness in different ethnic groups. Thus, different marker sets will likely be required for different ethnic groups in order to maximize their information content for linkage calculations.
我们使用190个平均间距为20厘摩（cM）的标记，对49对患有2型糖尿病的加拿大奥吉 - 克里族同胞对进行了全基因组扫描。其中四个标记，分别位于6号、8号、16号和22号染色体上，在奥吉 - 克里族中显示出与2型糖尿病的潜在连锁和潜在关联。这些标记均与目前在其他人群中已发现的与2型糖尿病相关的任何染色体区域或标记不对应。因此，在本研究样本中可能存在多个赋予2型糖尿病易感性的基因位点。我们正在通过增加这些连锁和关联区域内标记的密度以及增加研究对象的数量来跟进这些初步线索。此外，我们发现奥吉 - 克里族与作为对照的高加索人在标记杂合性方面存在很大差异。这表明，用于全基因组扫描的特定一组标记在不同种族群体中的信息量会有所不同。因此，为了在连锁计算中最大化信息含量，不同种族群体可能需要不同的标记集。
