一种与转移相关基因MTA1具有同源性的新型人类基因MTA1-L1的分子克隆、定位及特性分析
Molecular cloning, mapping, and characterization of a novel human gene, MTA1-L1, showing homology to a metastasis-associated gene, MTA1.
作者信息
Futamura M, Nishimori H, Shiratsuchi T, Saji S, Nakamura Y, Tokino T
机构信息
Laboratory of Molecular Medicine, University of Tokyo, Japan.
出版信息
J Hum Genet. 1999;44(1):52-6. doi: 10.1007/s100380050107.
Through large-scale sequencing of clones randomly selected from libraries of human cDNAs, we have isolated a novel human gene encoding a product with 59.6% identity in amino acid sequence to human MTA1, a protein associated with tumor invasion and metastasis. This cDNA, named MTA1-L1 (MTA1 like 1), consists of 2736 nucleotides with an open reading frame encoding 668 amino acids. A single 3.0-kb transcript of MTA1-L1 was expressed ubiquitously on Northern blots. Structural analysis of the MTA1-L1 gene revealed 18 exons spanning 8.1 kb of genomic DNA. We assigned the MTA1-L1 locus to chromosomal band 11q12-13.1 by fluorescence in situ hybridization.
通过对从人类cDNA文库中随机挑选的克隆进行大规模测序，我们分离出了一个新的人类基因，其编码产物的氨基酸序列与人类MTA1有59.6%的同一性，MTA1是一种与肿瘤侵袭和转移相关的蛋白质。这个cDNA被命名为MTA1-L1（MTA1样1），由2736个核苷酸组成，有一个编码668个氨基酸的开放阅读框。在Northern印迹上，MTA1-L1的一个单一3.0-kb转录本在各处均有表达。对MTA1-L1基因的结构分析显示，它有18个外显子，跨越8.1 kb的基因组DNA。我们通过荧光原位杂交将MTA1-L1基因座定位到染色体带11q12-13.1。
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