Dal Monte P, Varani S, Lazzarotto T, Pignatelli S, Landini M P
Department of Clinical and Experimental Medicine, University of Bologna, Italy.
Arch Virol. 1998;143(12):2413-9. doi: 10.1007/s007050050470.
This work demonstrates that antibodies to the product of the recombinant pUS22 of human cytomegalovirus (HCMV) are present in human sera during natural infection. US22 gene product has been identified as a member of the US22 family which may be secreted from infected cells. It is an early protein of 593 amino acids, 76 Kd in molecular weight. US22 seems to be an antigen which stimulates a good IgG response. In fact specific IgGs were found in approximately 40% of the CMV positive sera irrespective of their anti-CMV IgG titer. Specific IgM antibodies to pUS22 were observed exclusively during primary infection and in the sera with a high anti-CMV IgM titer. pUS22 could be considered for inclusion in a cocktail of CMV recombinant proteins to determine seropositivity to CMV and also to diagnose an active CMV infection.
这项研究表明，在自然感染期间，人血清中存在针对人巨细胞病毒（HCMV）重组pUS22产物的抗体。US22基因产物已被鉴定为US22家族的成员，该家族成员可能从受感染细胞中分泌。它是一种早期蛋白质，由593个氨基酸组成，分子量为76千道尔顿。US22似乎是一种能刺激产生良好IgG反应的抗原。事实上，在大约40%的CMV阳性血清中发现了特异性IgG，而不论其抗CMV IgG滴度如何。仅在原发性感染期间以及抗CMV IgM滴度高的血清中观察到针对pUS22的特异性IgM抗体。pUS22可考虑纳入CMV重组蛋白混合物中，以确定CMV血清阳性，也用于诊断活动性CMV感染。
