脉络膜黑色素瘤的DNA倍体模式：与生存的相关性。对存档材料的流式细胞术研究。
DNA ploidy pattern in choroidal melanoma: correlation with survival. A flow cytometry study on archival material.
作者信息
Toti P, Greco G, Mangiavacchi P, Bruni A, Palmeri M L, Luzi P
机构信息
Institute of Pathological Anatomy and Histology, University of Siena, Italy.
出版信息
Br J Ophthalmol. 1998 Dec;82(12):1433-7. doi: 10.1136/bjo.82.12.1433.
BACKGROUND/AIMS: Paraffin embedded samples have provided an important source of material for retrospective cytofluorimetric studies, useful in establishing the predictive value of DNA content measurements. The aim of this study was to investigate the incidence and type of aneuploidy in choroidal malignant melanomas (CMM) and the significance in the clinical outcome (median follow up 55 months).
METHODS
DNA content was quantified by flow cytometry in 61 CMM from archival material. Non-tumour ocular tissue was used as the reference diploid standard. Cases in which the coefficient of variation (CV) of the diploid peak was > 8% were excluded. The CMM were classified as spindle A, spindle B, mixed spindle and epithelioid, epithelioid, and necrotic.
RESULTS
The frequency of the aneuploid DNA pattern was 38%. Necrotic tumours showed a worse clinical outcome independent of the ploidy pattern. Spindle A tumours were found to be diploid. Spindle B and mixed tumours showed a prevalent diploid and near diploid aneuploid pattern (DI < 1.3), yet aneuploidy was not correlated with a worse prognosis. The epithelioid tumours were prevalently diploid. However, 83% of the aneuploid tumours were hypodiploid (DI < 0.95), and showed the worst prognosis.
CONCLUSION
These results indicate that increasing DNA abnormalities in CMM, especially in the epithelioid histotype, were associated with an increasing mortality.
背景/目的：石蜡包埋样本为回顾性细胞荧光分析研究提供了重要的材料来源，有助于确定DNA含量测量的预测价值。本研究旨在调查脉络膜恶性黑色素瘤（CMM）中非整倍体的发生率和类型，以及其对临床结局的意义（中位随访55个月）。
方法
采用流式细胞术对61例存档材料中的CMM进行DNA含量定量分析。以非肿瘤性眼组织作为参考二倍体标准。二倍体峰变异系数（CV）>8%的病例被排除。CMM分为梭形A、梭形B、梭形和上皮样混合、上皮样以及坏死型。
结果
非整倍体DNA模式的频率为38%。坏死性肿瘤显示出较差的临床结局，与倍体模式无关。梭形A肿瘤为二倍体。梭形B和混合型肿瘤以二倍体和近二倍体非整倍体模式为主（DI<1.3），但非整倍体与较差的预后无关。上皮样肿瘤以二倍体为主。然而，83%的非整倍体肿瘤为亚二倍体（DI<0.95），且预后最差。
结论
这些结果表明，CMM中DNA异常增加，尤其是上皮样组织学类型，与死亡率增加相关。
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