Richardson R P, Lawry L, Rees R C, Rennie I G
University Department of Ophthalmology and Orthoptics, Sheffield University, S. Yorks, UK.
Eye (Lond). 1997;11 ( Pt 5):629-34. doi: 10.1038/eye.1997.167.
In many different solid tumours the DNA content of cells has been reported to be associated with malignancy. With one exception, studies using flow cytometry to estimate cellular DNA in uveal melanoma have used archival material. We report the findings of a prospective study using fresh uveal melanoma where the DNA index (DI) and percentage of cells in S phase (%SPF) have been determined by flow cytometry in 51 patients with a minimum follow-up of 5 years. Forty-one (80%) of the tumours were large, 9 (18%) were medium and only 1 was small. Eight (16%) were epithelioid tumours, 41 (80%) were mixed cell and only 2 were spindle B cell tumours. Twenty-seven tumours were diploid, of which 10 (37%) developed metastases. Of the 24 aneuploid tumours, metastases developed in 6 (25%). The median %SPF was significantly reduced in patients with diploid tumours compared with aneuploid tumours (p < 0.0001), but there were no significant differences in %SPF between tumours associated with or without metastatic disease. The DI and %SPF were not significantly associated with the operation performed, cell morphology, tumour size or location. Neither %SPF nor aneuploidy was associated with a poor prognosis. Our results suggest that the estimation of DI or %SPF by flow cytometry is of little value in predicting survival from uveal melanomas after a minimum follow-up of 5 years.
据报道，在许多不同的实体瘤中，细胞的DNA含量与恶性程度相关。除了一个例外，使用流式细胞术估计葡萄膜黑色素瘤细胞DNA的研究都使用了存档材料。我们报告了一项前瞻性研究的结果，该研究使用新鲜的葡萄膜黑色素瘤，通过流式细胞术测定了51例患者的DNA指数（DI）和S期细胞百分比（%SPF），这些患者的最短随访时间为5年。其中41个（80%）肿瘤较大，9个（18%）为中等大小，只有1个较小。8个（16%）为上皮样肿瘤，41个（80%）为混合细胞型，只有2个为梭形B细胞肿瘤。27个肿瘤为二倍体，其中10个（37%）发生了转移。在24个非整倍体肿瘤中，6个（25%）发生了转移。与非整倍体肿瘤相比，二倍体肿瘤患者的%SPF中位数显著降低（p < 0.0001），但有转移或无转移疾病的肿瘤之间的%SPF没有显著差异。DI和%SPF与所进行的手术、细胞形态、肿瘤大小或位置均无显著关联。%SPF和非整倍体均与预后不良无关。我们的结果表明，在最短随访5年后，通过流式细胞术估计DI或%SPF对预测葡萄膜黑色素瘤的生存率价值不大。
