Fletcher T C, Jones R, Reid L
Histochem J. 1976 Nov;8(6):597-608. doi: 10.1007/BF01003961.
A quantitative analysis has been made of the glycoproteins present in the goblet cells of the epidermis, gill filaments and gill lamellae of three species of teleost fish. The glycoproteins have been identified by a combination of techniques, including the use of the enzyme sialidase followed by Alcian Blue staining, at PH 2.6 or 1.0, in combination with periodic acid-Schiff. The selected fish were representative of species living in marine, freshwater and estuarine environments. The range of glycoproteins identified in these fish was similar to that found in mammalian tissue in that both neutral and acid glycoproteins were present, the latter included both sialomucins sensitive and resistant to sialidase, and sulphomucin. A single goblet cell contained either neutral or acid glycoproteins alone or in combination. Only the epidermis of the plaice and rainbow trout contained uniform cell populations producing acid glycoproteins, the former sulphomucin and the latter mainly sialomucin. At each site in the flounder and in the gill epithelia of the plaice and rainbow trout, the goblet cell population was mixed, with cells producing each type of glycoprotein. The number of goblet cells producing each type of glycoprotein varied at each tissue site.
对三种硬骨鱼的表皮、鳃丝和鳃小片杯状细胞中的糖蛋白进行了定量分析。通过多种技术相结合的方法对糖蛋白进行了鉴定，包括在pH 2.6或1.0条件下使用唾液酸酶后进行阿尔辛蓝染色，并结合过碘酸-希夫反应。所选鱼类代表了生活在海洋、淡水和河口环境中的物种。在这些鱼类中鉴定出的糖蛋白范围与在哺乳动物组织中发现的相似，即同时存在中性和酸性糖蛋白，后者包括对唾液酸酶敏感和不敏感的唾液粘蛋白以及硫粘蛋白。单个杯状细胞仅含有中性或酸性糖蛋白，或两者兼而有之。只有鲽鱼和虹鳟鱼的表皮含有产生酸性糖蛋白的均匀细胞群体，前者为硫粘蛋白，后者主要为唾液粘蛋白。在比目鱼以及鲽鱼和虹鳟鱼的鳃上皮的每个部位，杯状细胞群体都是混合的，有产生每种类型糖蛋白的细胞。在每个组织部位，产生每种类型糖蛋白的杯状细胞数量各不相同。
