Matzke K H, Thiessen G
Histochemistry. 1976 Oct 7;49(1):73-9. doi: 10.1007/BF00490128.
The present investigation was designed to allow a critical comparison of the dye purity of six commercial acriflavine samples. Thin layer chromatography, absorption-, IR- and NMR-spectroscopy were applied for the identification of dye components and impurities. Ambiguities regarding the purity of the acriflavine samples have been resolved, showing that: (a) The finding permits the conclusion, that all analyzed samples of the fluorochrome "acriflavine" are characterized by a two-component dye pattern (acriflavine II and proflavine III), and contain fluorescent impurities. (b) The dye component III was the main component of only one dye sample. The effectiveness of these experiments is concerned with making automated microfluorometric measurement of cells stained with pure dye fractions more quantitative and reproduceable.
本次研究旨在对六个市售吖啶黄样品的染料纯度进行严格比较。采用薄层色谱法、吸收光谱法、红外光谱法和核磁共振光谱法来鉴定染料成分和杂质。吖啶黄样品纯度方面的模糊问题已得到解决，结果表明：(a) 这一发现使得可以得出结论，即荧光染料“吖啶黄”的所有分析样品均具有双组分染料模式（吖啶黄II和原黄素III），并含有荧光杂质。(b) 染料成分III只是其中一个染料样品的主要成分。这些实验的成效在于使对用纯染料组分染色的细胞进行自动显微荧光测量更加定量且可重复。
