Purcell J M, Quimby D J
J Assoc Off Anal Chem. 1976 Nov;59(6):1251-4.
A new rapid method for the quantitative and routine determination of free amino groups in intact pure proteins has been developed. Primary amino groups are labeled with fluorescamine and the labeled groups are detected by absorption spectroscopy in the range 375-390 nm. The amino group concentration can be determined in a few minutes without hydrolyzing the labeled protein and extracting a lysine derivative. The method was tested with the following proteins: lysozyme, alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin, ribonuclease, ribonuclease-S-peptide, and alphasl-casein B. Application of this method to the estimation of available lysine is discussed.
已开发出一种用于完整纯蛋白质中游离氨基定量和常规测定的新快速方法。伯氨基用荧光胺标记，标记基团通过375 - 390 nm范围内的吸收光谱进行检测。无需水解标记的蛋白质并提取赖氨酸衍生物，即可在几分钟内测定氨基浓度。该方法已用以下蛋白质进行了测试：溶菌酶、α-乳白蛋白、β-乳球蛋白、牛血清白蛋白、核糖核酸酶、核糖核酸酶-S-肽和αs1-酪蛋白B。讨论了该方法在可利用赖氨酸估计中的应用。
