Patthy M, Patthy A
Acta Biochim Biophys Acad Sci Hung. 1975;10(4):277-86.
A simple procedure was worked out for the direct spectrophotometric determination of lysine and/or ornithine in hydrolysates of proteins and protein-containing materials. The method is based on the estimation of the coloured compound formed in the reaction of lysine and ornithine with furfurol. Since the reaction is specific for lysine and ornithine and separation of amino acids is not necessary for their determination the method is suitable for large scale screening of protein samples. The lower limit of detection is 0.5 mug for lysine and 2 mug for ornithine and the accuracy of the method is +/- 0.5% and +/- 2% respectively.
已制定出一种简单的程序，用于直接分光光度法测定蛋白质及含蛋白质材料水解产物中的赖氨酸和/或鸟氨酸。该方法基于对赖氨酸和鸟氨酸与糠醛反应形成的有色化合物的估算。由于该反应对赖氨酸和鸟氨酸具有特异性，且测定时无需分离氨基酸，因此该方法适用于蛋白质样品的大规模筛选。赖氨酸的检测下限为0.5微克，鸟氨酸为2微克，该方法的准确度分别为±0.5%和±2%。
